Zoom 4321F Bedienungsanleitung PDF herunterladen (Seite 2)

Built-in epi illumination sources: 365nm UV,

460-470nm blue and broadband white light

Motorized five-position emission filter

wheel with EtBr, SYBR Green and SYBR

Gold filters; interchangeable standard and

custom filters available

LED white light illuminator provides uniform

background light for colonies, Coomassie Blue

and Silver stained gels; place the tray in the

side pocket when the illuminator is not in use

VisionWorks

LS software allows image

acquisition, enhancement, documentation,

printing, publishing and analysis

BioSpectrum Darkroom

is light tight and automated

with all functions controlled

via VisionWorksLS software

Quickly adjust the motorized platform

tray height to any position (a manual lift

platform is also available)

Systems can be configured with a

high specification 20” touch screen

computer/monitor (order separately)

MegaCam 810 Camera OptiChemi 610 Camera BioChemi 510 Camera GelCam 310 Camera

8.1 megapixel resolution

scientific grade CCD with

regulated cooling

technology to capture

the finest details in gels and

blots to achieve accurate

quantitative analysis

3.2 mepixels resolution

scientific grade CCD,

extendable to 9.6MP,

deeply cooled for extended

exposure times in IR/NIR

multiplex and

chemiluminescent imaging

2.1 megapixel resolution

scientific grade CCD,

extendable to 7.4MP, with

regulated cooling and low

noise for imaging a variety of

chemiluminescent

and fluorescent samples

2.0 megapixel resolution

scientific grade CCD,

extendable to 6.0MP,

provides the highest

resolution camera for

general purpose fluorescent

and colorimetric samples

Unique gel viewer window

blocks UV while allowing

visualization of samples

without opening the

darkroom door

Access port for connecting

to the external BioLite high

intensity multispectral light

source (optional)

Scientific Grade CCD Camera mounted in the darkroom and protected by the camera cover

BioSpectrum Imaging System

VisionWorksLS is a sophisticated image capture and analysis

software package with comprehensive tools to facilitate the capture

of chemiluminescent, fluorescent or colorimetric stained gels, blots,

colonies and membranes. Capabilities include:

Extensive image acquisition functions

Image enhancement capabilities

1D and area density analysis plus colony counting

One-touch automated macros

User-defined templates and preference settings

Support for 21 CFR Part 11 compliance

Report generation and export of data to Excel

VisionWorksLS software offers many image enhancement features, process filters and

annotation capabilities as non-destructive tools for visualization and publication.

Annotation can be added in the form of text,

lines, highlights and more

Filter tools include align, rotate, emboss,

sharpen, resize, starfield subtraction and

background correction

Spatial calibration determines image scale

and measure lengths, angles and areas

Annotation. Overlay non-destructive

annotation or “burn” the annotation into

an image for permanent documentation.

Camera Control and Image

Capture. Camera menu guides

the user through the easy-to-

use image acquisition steps.

Software Control. User-friendly, one-touch simplicity for

automating image capture and analysis!

UVP’s cameras are selected for their high resolution and sensitivity for image capture as well as

for the ability to control the capture settings. The integrated software menu allows selection of

functions to achieve superior captured images.

Integration functions include on-chip integration for the simplest image capture. Sequential

integration captures multiple pictures taken at a uniformly increasing exposure time.

Dynamic integration captures images at set intervals.

Binning allows a quick preview of the image before continuing with a longer full resolution

exposure.

Saturation preview assures imaging results are quantifiable by detecting over-exposure of

bands in live preview.

Imaging templates allow for creation of custom settings to enable quick image capture with

reproducible results.

AutoExpose enables the perfect image exposure to be captured automatically below the

saturation level of every pixel in the image for the widest dynamic range

possible and the best quantitative analysis of bands.

Image Capture Capabilities

Image Enhancement Tools

VisionWorks

LS Software

Imaging Made Easy for

Chemiluminescence | Bioluminescence | Colorimetric | Fluorescence

Benefits of the BioSpectrum

Select from single or multiple

UV wavelength Benchtop

Transilluminators

Alexa PAGE GelGreen/GelRed Ethidium Bromide Autoradiograph Multiplex NIR2D Silver Stain Chemi Western BlotColonies Chemi DNA Northern Blot

Emission and

Excitation Filters

Expand the BioSpectrum’s

imaging capabilities by

adding filters for specific

applications. Refer to UVP’s

filter chart for details.

Motorized

Platform

Height adjusts to

any location in a

ten inch travel

range.

Chemi Tray

Use with Western

blots or other

bioluminescent or

chemiluminescent

samples.

The BioSpectrum Imaging System enables multiple applications including imaging of chemiluminescent

(Westerns, Northerns and Southerns), fluorescent, bioluminescent and colorimetric samples. Application Notes

and referenced articles can be accessed from UVP’s web site. Two salinet imaging applications are noted:

Featured Applications

Feature Benefit

Scientific Grade Cameras

Choice of CCD cameras for high resolution, high quality images. Select a camera

based on the imaging applications (refer to the camera list on page 2).

Motorized Lenses Automated control of lens for quick image acquisition and rapid processing.

Motorized Platform

Height adjustable platform moves to any position in a 10 inch range for

optimized image capture. Platform is easily controlled via the software interface.

LED White Light Illuminator Extremely high uniformity for minimal image enhancement.

Viewing Window View the sample through the UV safe window without opening the darkroom door.

Light Tight Darkroom Creates optimum conditions for imaging gels and blots.

Chemi Tray Place and capture bioluminescent or chemiluminescent samples.

Epi Illumination Sources Built in illumination sources for positioning and exciting samples from above.

Access Ports

Connect numerous external sources to enhance imaging techniques

and acquisition.

Motorized Filter Wheel

Simplify imaging by controlling the filter wheel through a software button.

Programmable filter wheel enables automatic selection of the appropriate filter for

a specific application.

Multiplex Imaging

VisionWorksLS permits multiplexing so that several proteins in a sample can be detected and analyzed at the

same time on a single protein blot. NIR labels, in particular, offer very low background and high signal-to-noise

ratio for quantitative imaging. The combination of the BioLite MultiSpectral Light Source and BioSpectrum

Imaging System provides a full range of wavelengths for excitation light and offers rapid, high resolution image

capture through the use of cooled CCD cameras and low light lenses. Typically, exposures are complete in

30 seconds to 2 minutes. Images are captured and processed with VisionWorks LS image acquisition and

analysis software to composite the pseudocolored images.

Chemiluminescent imaging of protein blots using the BioSpectrum greatly speeds up

and simplifies analysis. Once positioned on the imaging platform, the membrane is

focused, door closed and the image captured with a one button preset in the

VisionWorksLS software. Multiple captures are easily taken to acquire a full range of

exposures using the Sequential Integration feature. Chemiluminescent imaging of

protein blotting and the sensitivity of this procedure compares to film showing the

BioSpectrum with cooled CCD camera to be superior to film in sensitivity, accuracy,

dynamic range, speed, and simplicity. Due to the high sensitivity and resolution of the

BioSpectrum system, the resultant image is both quantitative and publication quality.

Chemiluminescent Imaging

Chemiluminescent blots comparing film to

CCD capture shows the BioSpectrum 500 is

more sensitive than film.

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Zoom 4321F Bedienungsanleitung Seite 2

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